基因数据处理32之Avocado运行记录(人造数据集)

主要是需要数据正确，如果中间缺少记录，avocado一般不会成功
1.代码：
Avocado修改：

/*** Licensed to Big Data Genomics (BDG) under one* or more contributor license agreements.  See the NOTICE file* distributed with this work for additional information* regarding copyright ownership.  The BDG licenses this file* to you under the Apache License, Version 2.0 (the* "License"); you may not use this file except in compliance* with the License.  You may obtain a copy of the License at** http://www.apache.org/licenses/LICENSE-2.0** Unless required by applicable law or agreed to in writing, software* distributed under the License is distributed on an "AS IS" BASIS,* WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.* See the License for the specific language governing permissions and* limitations under the License.*/
package org.bdgenomics.avocado.cliimport java.nio.file.Files
import org.apache.commons.configuration.HierarchicalConfiguration
import org.apache.commons.configuration.plist.PropertyListConfiguration
import org.apache.hadoop.mapreduce.Job
import org.apache.spark.rdd.RDD
import org.apache.spark.{SparkContext, Logging}
import org.kohsuke.args4j.{Option => option, Argument}
import org.bdgenomics.adam.models.{VariantContext, ReferenceRegion}
import org.bdgenomics.adam.rdd.ADAMContext._
import org.bdgenomics.avocado.Timers._
import org.bdgenomics.avocado.discovery.Explore
import org.bdgenomics.avocado.genotyping.CallGenotypes
import org.bdgenomics.avocado.input.Input
import org.bdgenomics.avocado.models.Observation
import org.bdgenomics.avocado.preprocessing.Preprocessor
import org.bdgenomics.avocado.postprocessing.Postprocessor
import org.bdgenomics.avocado.stats.AvocadoConfigAndStats
import org.bdgenomics.formats.avro.{
Variant,
AlignmentRecord,
NucleotideContigFragment,
Genotype
}
import org.bdgenomics.utils.cli.{
BDGSparkCommand,
BDGCommandCompanion,
ParquetArgs,
Args4j,
Args4jBase
}
import org.bdgenomics.utils.instrumentation._object Avocado extends BDGCommandCompanion {val commandName = "Avocado"val commandDescription = "Call variants using avocado and the ADAM preprocessing pipeline."def apply(args: Array[String]) = {new Avocado(Args4j[AvocadoArgs](args))}
}class AvocadoArgs extends Args4jBase with ParquetArgs {@Argument(metaVar = "READS", required = true, usage = "ADAM read-oriented data", index = 0)var readInput: String = _@Argument(metaVar = "REFERENCE", required = true, usage = "ADAM or FASTA reference genome data", index = 1)var referenceInput: String = _@Argument(metaVar = "VARIANTS", required = true, usage = "ADAM variant output", index = 2)var variantOutput: String = _@Argument(metaVar = "CONFIG", required = true, usage = "avocado configuration file", index = 3)var configFile: String = _@option(name = "-debug", usage = "If set, prints a higher level of debug output.")var debug = false@option(required = false, name = "-fragment_length", usage = "Sets maximum fragment length. Default value is 10,000. Values greater than 1e9 should be avoided.")var fragmentLength: Long = 10000L
}class Avocado(protected val args: AvocadoArgs) extends BDGSparkCommand[AvocadoArgs] with Logging {// companion object to this class - needed for BDGCommand frameworkval companion = Avocado/** *********add by xubo 20160528 *******************/println("companion:" + companion)println("companion.commandName:" + companion.commandName)println("companion.commandDescription:" + companion.commandDescription)//  println("companion.commandName:"+companion.)println("AvocadoArgs:")println("args:" + args)println("args.configFile:" + args.configFile)println("args.debug:" + args.debug)println("args.fragmentLength:" + args.fragmentLength)println("args.readInput:" + args.readInput)println("args.referenceInput:" + args.referenceInput)println("args.variantOutput:" + args.variantOutput)println("test end\n")/** *********add by xubo 20160528 *******************/// get config off classpath and load into a temp file...val stream = Thread.currentThread.getContextClassLoader.getResourceAsStream(args.configFile)val tempPath = Files.createTempDirectory("config")val tempFilePath = tempPath.resolve("temp.properties")Files.copy(stream, tempFilePath)/** *********add by xubo 20160528 *******************/println("stream:" + stream)println("tempFilePath:" + tempFilePath)/** *********add by xubo 20160528 *******************/// load configval config: HierarchicalConfiguration = new PropertyListConfiguration(tempFilePath.toFile)val preprocessorNames = getStringArrayFromConfig("preprocessorNames")val preprocessorAlgorithms = getStringArrayFromConfig("preprocessorAlgorithms")assert(preprocessorNames.length == preprocessorAlgorithms.length,"Must have a 1-to-1 mapping between preprocessor names and algorithms.")val preprocessingStagesZippedWithNames = preprocessorNames.zip(preprocessorAlgorithms)val explorerName = config.getString("explorerName")val explorerAlgorithm = config.getString("explorerAlgorithm")val genotyperName = config.getString("genotyperName")val genotyperAlgorithm = config.getString("genotyperAlgorithm")val postprocessorNames = getStringArrayFromConfig("postprocessorNames")val postprocessorAlgorithms = getStringArrayFromConfig("postprocessorAlgorithms")assert(postprocessorNames.length == postprocessorAlgorithms.length,"Must have a 1-to-1 mapping between postprocessor names and algoritms.")val postprocessorsZipped = postprocessorNames.zip(postprocessorAlgorithms)val debug = args.debug/** *********add by xubo 20160528 *******************/println("config:" + config)println("preprocessorNames:" + preprocessorNames)preprocessorNames.foreach(println)println("preprocessorAlgorithms:" + preprocessorAlgorithms)preprocessorAlgorithms.foreach(println)println("preprocessingStagesZippedWithNames:" + preprocessingStagesZippedWithNames)preprocessorNames.foreach(println)println("explorerName:" + explorerName)println("explorerAlgorithm:" + explorerAlgorithm)println("genotyperName:" + genotyperName)println("genotyperAlgorithm:" + genotyperAlgorithm)println("postprocessorNames:" + postprocessorNames)postprocessorNames.foreach(println)println("postprocessorAlgorithms:" + postprocessorAlgorithms)postprocessorAlgorithms.foreach(println)println("postprocessorsZipped:" + postprocessorsZipped)postprocessorsZipped.foreach(println)println("stream:" + stream)println("stream:" + stream)println("stream:" + stream)println("stream:" + stream)/** *********add by xubo 20160528 *******************/private def getStringArrayFromConfig(name: String): Array[String] = {config.getStringArray(name).map(_.toString)}/*** Applies several pre-processing steps to the read pipeline. Currently, these are the default* steps in the ADAM processing pipeline.** @param reads RDD of reads to process.* @return RDD containing reads that have been sorted and deduped.*/def preProcessReads(reads: RDD[AlignmentRecord]): RDD[AlignmentRecord] = PreprocessReads.time {var processedReads = reads //.cacheif (debug) {log.info("avocado: Preprocessing " + processedReads.count + " reads.")}// loop over preprocessing stages and applypreprocessingStagesZippedWithNames.foreach(p => {val (stageName, stageAlgorithm) = plog.info("avocado: Running " + stageName)// run this preprocessing stageprocessedReads = Preprocessor(processedReads, stageName, stageAlgorithm, config)})// return processed readsprocessedReads}/*** Applies variant calling algorithms to reads and pileups. Reduces down and returns called variants.** @param reads* @param stats* @return Joined output of variant calling algorithms.*/def callVariants(reads: RDD[AlignmentRecord], stats: AvocadoConfigAndStats): RDD[VariantContext] = CallVariants.time {val discoveries: RDD[Observation] = Explore(explorerAlgorithm,explorerName,reads,stats,config)CallGenotypes(genotyperAlgorithm,genotyperName,discoveries,stats,config)}/*** Applies variant post-processing methods to called variants. Post-processing can* include methods which modify the information in variant calls, or alternatively,* methods that filter out spurious variant calls.** @param variants RDD of variants to process.* @return Post-processed variants.*/def postProcessVariants(variants: RDD[VariantContext], stats: AvocadoConfigAndStats): RDD[VariantContext] = PostprocessVariants.time {var rdd = variants// loop over post processing stepspostprocessorsZipped.foreach(p => {val (ppStageName, ppAlgorithm) = prdd = Postprocessor(rdd, ppStageName, ppAlgorithm, stats, config)})rdd}/*** Main method. Implements body of variant caller. SparkContext and Hadoop Job are provided* by the ADAMSparkCommand shell.** @param sc  SparkContext for RDDs.* @param job Hadoop Job container for file I/O.*/def run(sc: SparkContext) {log.info("Starting avocado...")// load in reference from ADAM fileval reference: RDD[NucleotideContigFragment] = LoadContigs.time {sc.loadSequence(args.referenceInput, fragmentLength = args.fragmentLength)}log.info("Loading reads in from " + args.readInput)// load in reads from ADAM fileval reads: RDD[AlignmentRecord] = LoadReads.time {Input(sc, args.readInput, reference, config)}/** *********add by xubo 20160528 *******************/println("readInput:")reads.foreach(println)/** *********add by xubo 20160528 *******************/// create stats/config itemval stats = new AvocadoConfigAndStats(sc, args.debug, reads, reference)/** *********add by xubo 20160528 *******************/println("stats:" + stats)println("stats.contigLengths:" + stats.contigLengths)//    println("stats.coverage:" + stats.coverage)println("stats.debug:" + stats.debug)println("stats.referenceObservations:" + stats.referenceObservations)//    println("stats.referenceSeq:" + stats.referenceSeq)//    stats.referenceSeq.foreach(println)println("stats.samplesInDataset:" + stats.samplesInDataset)stats.samplesInDataset.foreach(println)println("stats.sequenceDict:" + stats.sequenceDict)//    println("stats.contigLengths:"+stats)/** *********add by xubo 20160528 *******************/// apply read translation stepslog.info("Processing reads.")var cleanedReads = preProcessReads(reads)/** *********add by xubo 20160528 *******************/println("cleanedReads:" + cleanedReads)println("cleanedReads.count:" + cleanedReads.count())cleanedReads.foreach(println)val cleanedReads2 = cleanedReads.map { each =>each.setRecordGroupSample("hello")each}//    cleanedReads.adamGetReadGroupDictionary()//    cleanedReads.getSequenc//    cleanedReads.foreach(each => println(each.getSequence + " " + each.referenceLength))println("cleanedReads2:")cleanedReads2.foreach(println)/** *********add by xubo 20160528 *******************/// call variants on filtered reads and pileupslog.info("Calling variants.")
//    val calledVariants = callVariants(cleanedReads, stats)val calledVariants = callVariants(cleanedReads2, stats)/** *********add by xubo 20160528 *******************/println("calledVariants:" + calledVariants)println("calledVariants.count:" + calledVariants.count())//        calledVariants.take(10).foreach(each=>println(each.databases+" "+each.position+" "+each.variant+" "=each.genotypes))//    calledVariants.take(10).foreach(println)/** *********add by xubo 20160528 *******************/// post process variantslog.info("Post-processing variants.")val processedGenotypes: RDD[Genotype] = postProcessVariants(calledVariants, stats).flatMap(variantContext => variantContext.genotypes)/** *********add by xubo 20160528 *******************/println("processedGenotypes:" + calledVariants)println("processedGenotypes.count:" + processedGenotypes.count())processedGenotypes.foreach(println)/** *********add by xubo 20160528 *******************/// save variants to output filelog.info("Writing calls to disk.")SaveVariants.time {processedGenotypes.adamParquetSave(args.variantOutput,args.blockSize,args.pageSize,args.compressionCodec,args.disableDictionaryEncoding)/** ***********add ***************/processedGenotypes.foreach { each => println("processedGenotypes:" + each) }//      processedGenotypes.}}
}

AvocadoSuite没有上传，在个人idea的package org.bdgenomics.avocado.cli中
数据为：

val samFile = "artificial.realigned.sam"val faFile = "artificial.fa"

2.运行记录;

D:\1win7\java\jdk\bin\java -Didea.launcher.port=7532 "-Didea.launcher.bin.path=D:\1win7\idea\IntelliJ IDEA Community Edition 15.0.4\bin" -Dfile.encoding=UTF-8 -classpath 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IDEA Community Edition 15.0.4\lib\idea_rt.jar" com.intellij.rt.execution.application.AppMain org.bdgenomics.avocado.cli.AvocadoSuite
SLF4J: Class path contains multiple SLF4J bindings.
SLF4J: Found binding in [jar:file:/D:/1win7/java/apache-maven-3.3.9-bin/Repository/org/slf4j/slf4j-log4j12/1.7.12/slf4j-log4j12-1.7.12.jar!/org/slf4j/impl/StaticLoggerBinder.class]
SLF4J: Found binding in [jar:file:/D:/1win7/java/apache-maven-3.3.9-bin/Repository/org/bdgenomics/adam/adam-cli_2.10/0.18.2/adam-cli_2.10-0.18.2.jar!/org/slf4j/impl/StaticLoggerBinder.class]
SLF4J: See http://www.slf4j.org/codes.html#multiple_bindings for an explanation.
SLF4J: Actual binding is of type [org.slf4j.impl.Log4jLoggerFactory]
2016-05-28 18:42:08 WARN  NativeCodeLoader:62 - Unable to load native-hadoop library for your platform... using builtin-java classes where applicable
/D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/artificial.realigned.sam
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 0, "oldPosition": 10, "end": 70, "mapq": 100, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "34M10D26M", "oldCigar": "44M10D16M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:11\tNM:i:10\tRG:Z:read_group_id\tOC:Z:44M10D16M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 110, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 5, "oldPosition": null, "end": 75, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "29M10D31M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "29^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G11", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 105, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 10, "oldPosition": 20, "end": 80, "mapq": 100, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "24M10D36M", "oldCigar": "34M10D26M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:21\tNM:i:10\tRG:Z:read_group_id\tOC:Z:34M10D26M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 120, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 15, "oldPosition": null, "end": 85, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "19M10D41M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "19^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G21", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 115, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 25, "oldPosition": null, "end": 95, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "9M10D51M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "9^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G31", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 125, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 105, "oldPosition": null, "end": 165, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 5, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 110, "oldPosition": null, "end": 170, "mapq": 90, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 0, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 115, "oldPosition": null, "end": 175, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 15, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 120, "oldPosition": null, "end": 180, "mapq": 90, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 10, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 125, "oldPosition": null, "end": 185, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 25, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
faLoad:
/D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/artificial.fa
{"contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": null}, "description": "fasta", "fragmentSequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "fragmentNumber": 0, "fragmentStartPosition": 0, "fragmentLength": 1120, "numberOfFragmentsInContig": 1}
C:\Users\xubo\AppData\Local\Temp\83178325081869260/var
/D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/basic.properties
(%s,123)
D:\all\idea\avocado-master\avocado-cli\src\test\resources\output\var20160528184213248
companion:org.bdgenomics.avocado.cli.Avocado$@3e4e92e3
companion.commandName:Avocado
companion.commandDescription:Call variants using avocado and the ADAM preprocessing pipeline.
AvocadoArgs:
args:org.bdgenomics.avocado.cli.AvocadoArgs@188d950a
args.configFile:basic.properties
args.debug:false
args.fragmentLength:10000
args.readInput:/D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/artificial.realigned.sam
args.referenceInput:/D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/artificial.fa
args.variantOutput:D:\all\idea\avocado-master\avocado-cli\src\test\resources\output\var20160528184213248
test endstream:java.io.BufferedInputStream@7733d01d
tempFilePath:C:\Users\xubo\AppData\Local\Temp\config4218631409097485876\temp.properties
config:org.apache.commons.configuration.plist.PropertyListConfiguration@305e0ade
preprocessorNames:[Ljava.lang.String;@6e1b8da0
preprocessorAlgorithms:[Ljava.lang.String;@58424e18
preprocessingStagesZippedWithNames:[Lscala.Tuple2;@6fc8f5d9
explorerName:readExplorer
explorerAlgorithm:ReadExplorer
genotyperName:biallelicGenotyper
genotyperAlgorithm:BiallelicGenotyper
postprocessorNames:[Ljava.lang.String;@65fcd178
nonRef
postprocessorAlgorithms:[Ljava.lang.String;@7230adcb
filterReferenceCalls
postprocessorsZipped:[Lscala.Tuple2;@deacdcc
(nonRef,filterReferenceCalls)
stream:java.io.BufferedInputStream@7733d01d
stream:java.io.BufferedInputStream@7733d01d
stream:java.io.BufferedInputStream@7733d01d
stream:java.io.BufferedInputStream@7733d01d
Loading reads in from /D:/all/idea/avocado-master/avocado-cli/target/scala-2.10.3/test-classes/artificial.realigned.sam
readInput:
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 0, "oldPosition": 10, "end": 70, "mapq": 100, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "34M10D26M", "oldCigar": "44M10D16M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:11\tNM:i:10\tRG:Z:read_group_id\tOC:Z:44M10D16M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 110, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 5, "oldPosition": null, "end": 75, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "29M10D31M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "29^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G11", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 105, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 10, "oldPosition": 20, "end": 80, "mapq": 100, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "24M10D36M", "oldCigar": "34M10D26M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:21\tNM:i:10\tRG:Z:read_group_id\tOC:Z:34M10D26M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 120, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 15, "oldPosition": null, "end": 85, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "19M10D41M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "19^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G21", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 115, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 25, "oldPosition": null, "end": 95, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "9M10D51M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "9^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G31", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 125, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 105, "oldPosition": null, "end": 165, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 5, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 110, "oldPosition": null, "end": 170, "mapq": 90, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 0, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 115, "oldPosition": null, "end": 175, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 15, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 120, "oldPosition": null, "end": 180, "mapq": 90, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 10, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 125, "oldPosition": null, "end": 185, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 25, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
stats:org.bdgenomics.avocado.stats.AvocadoConfigAndStats@32b9c6f7
stats.contigLengths:Map(artificial -> 1120)
stats.debug:false
stats.referenceObservations:MapPartitionsRDD[26] at flatMap at SliceReference.scala:28
stats.samplesInDataset:[Ljava.lang.String;@551a9433
sequencing_center
stats.sequenceDict:SequenceDictionary{
artificial->1120}
cleanedReads:MapPartitionsRDD[21] at map at ADAMContext.scala:289
cleanedReads.count:10
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 0, "oldPosition": 10, "end": 70, "mapq": 100, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "34M10D26M", "oldCigar": "44M10D16M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:11\tNM:i:10\tRG:Z:read_group_id\tOC:Z:44M10D16M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 110, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 5, "oldPosition": null, "end": 75, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "29M10D31M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "29^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G11", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 105, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 10, "oldPosition": 20, "end": 80, "mapq": 100, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "24M10D36M", "oldCigar": "34M10D26M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:21\tNM:i:10\tRG:Z:read_group_id\tOC:Z:34M10D26M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 120, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 15, "oldPosition": null, "end": 85, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "19M10D41M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "19^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G21", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 115, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 25, "oldPosition": null, "end": 95, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "9M10D51M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "9^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G31", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 125, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 105, "oldPosition": null, "end": 165, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 5, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 110, "oldPosition": null, "end": 170, "mapq": 90, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 0, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 115, "oldPosition": null, "end": 175, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 15, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 120, "oldPosition": null, "end": 180, "mapq": 90, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 10, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 125, "oldPosition": null, "end": 185, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "sequencing_center", "mateAlignmentStart": 25, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
cleanedReads2:
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 0, "oldPosition": 10, "end": 70, "mapq": 100, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "34M10D26M", "oldCigar": "44M10D16M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:11\tNM:i:10\tRG:Z:read_group_id\tOC:Z:44M10D16M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 110, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 5, "oldPosition": null, "end": 75, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "29M10D31M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "29^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G11", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 105, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 10, "oldPosition": 20, "end": 80, "mapq": 100, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "24M10D36M", "oldCigar": "34M10D26M", "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": null, "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tOP:i:21\tNM:i:10\tRG:Z:read_group_id\tOC:Z:34M10D26M", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 120, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 111}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 15, "oldPosition": null, "end": 85, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "19M10D41M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "19^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G21", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 115, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 0, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 25, "oldPosition": null, "end": 95, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "9M10D51M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "9^GGGGGGGGGG10G0G0G0G0G0G0G0G0G0G31", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:20\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 125, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": 101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 105, "oldPosition": null, "end": 165, "mapq": 90, "readName": "read1", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 5, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 110, "oldPosition": null, "end": 170, "mapq": 90, "readName": "read2", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 0, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 115, "oldPosition": null, "end": 175, "mapq": 90, "readName": "read3", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 15, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 120, "oldPosition": null, "end": 180, "mapq": 90, "readName": "read4", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:100\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 10, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -111}
{"readNum": 1, "contig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "start": 125, "oldPosition": null, "end": 185, "mapq": 90, "readName": "read5", "sequence": "AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA", "qual": "IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII", "cigar": "60M", "oldCigar": null, "basesTrimmedFromStart": 0, "basesTrimmedFromEnd": 0, "readPaired": true, "properPair": true, "readMapped": true, "mateMapped": true, "failedVendorQualityChecks": false, "duplicateRead": false, "readNegativeStrand": false, "mateNegativeStrand": false, "primaryAlignment": true, "secondaryAlignment": false, "supplementaryAlignment": false, "mismatchingPositions": "60", "origQual": null, "attributes": "XS:i:70\tAS:i:70\tMQ:i:90\tNM:i:0\tRG:Z:read_group_id", "recordGroupName": "read_group_id", "recordGroupSequencingCenter": null, "recordGroupDescription": null, "recordGroupRunDateEpoch": null, "recordGroupFlowOrder": null, "recordGroupKeySequence": null, "recordGroupLibrary": "library", "recordGroupPredictedMedianInsertSize": null, "recordGroupPlatform": "illumina", "recordGroupPlatformUnit": "platform_unit", "recordGroupSample": "hello", "mateAlignmentStart": 25, "mateAlignmentEnd": null, "mateContig": {"contigName": "artificial", "contigLength": 1120, "contigMD5": null, "referenceURL": null, "assembly": null, "species": null, "referenceIndex": 0}, "inferredInsertSize": -101}
calledVariants:InstrumentedRDD[41] at mapPartitions at BiallelicGenotyper.scala:398
calledVariants.count:1110
processedGenotypes:InstrumentedRDD[41] at mapPartitions at BiallelicGenotyper.scala:398
processedGenotypes.count:11
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SLF4J: Failed to load class "org.slf4j.impl.StaticLoggerBinder".
SLF4J: Defaulting to no-operation (NOP) logger implementation
SLF4J: See http://www.slf4j.org/codes.html#StaticLoggerBinder for further details.
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read parquet:
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*************end*************Process finished with exit code 0

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